Problems of Channel Correlation and Statistical Bias in Photon-Correlation Spectroscopy

Correlation between channels of the normalized photocount-rate correlation function g(2)(τ) becomes significant at high count rates and leads to a number of data-analysis problems. We derive an expression for channel correlation that is valid for a detector area of arbitrary extent and compare the theoretical predictions with measured values. A data-analysis procedure is demonstrated that employs the theoretical expression for channel correlation and provides a rigorous test of an assumed fitting function. The procedure facilitates the use of the cumulant method in determining the polydispersity of scatterers. An expression for the statistical bias of g(2)(τ) is also derived and compared with measured values

Precision Management of Fruit Trees

The aim of the Special Issue “Precision Management of Fruit Trees” was to collect new insights to support the adoption of advanced, efficient, and sustainable management techniques in the fruit production sector. Indeed, this is an opportunity offered by the technological innovations adopted using new-generation sensors and implemented through precise management operations. This Special Issue contains 11 scientific articles contributing to our knowledge on the precision management of fruit trees, indicating the high activity of this sector and possibly leading to the application of new techniques/protocols to overcome global and rapidly changing environmental issues. Scalisi et al. [1], in their study, aimed to (i) determine the reliability of a portable Bluetooth colour meter for fruit colour measurements; (ii) characterise the changes in quantitative skin colour attributes in a nectarine cultivar in response to time from harvest; and (iii) determine the influence of row orientation and training system on nectarine skin colour. Overall, the device proved reliable for fruit colour detection. The results of this study highlight the potential of one of the measured parameters as a quantitative index to monitor ripening prior to harvest in nectarines. Remote sensing techniques based on images acquired from unmanned aerial vehicles (UAVs) could represent an effective tool to speed up the data acquisition process in phenotyping trials and, consequently, to reduce the time and cost of the field work. Caruso et al. [2] confirmed the ability of a UAV equipped with RGB-NIR cameras to highlight differences in geometrical and spectral canopy characteristics between eight olive cultivars planted at different planting distances in a hedgerow olive orchard. Tree densities have increased greatly in olive orchards over the last few decades. Ladux et al. [3], in their study, found that the leaf area index (LAI) of neighbouring trees modifies the light quality environment prior to a tree being directly shaded, as well as the morphological responses of olive cultivars to changes in light quality. The results suggested that cultivar differences in response to light quality may be relevant for understanding adaptation to dense orchards and identifying cultivars best suited to them. Saha et al. [4] found that monitoring plant vegetative growth can provide the basis for precise crop management. In this study, a 2D light detection and ranging (LiDAR) laser scanner, mounted on a linear conveyor, was used to acquire multi-temporal, three-dimensional (3D) data from strawberry plants. The results contributed to building up an approach for estimating plant geometrical features, particularly strawberry canopy volume profile based on LiDAR point cloud for tracking plant growth. Carella et al. [5] studied the physiological and productive behaviour of different olive cultivars grown under a high-density hedgerow system and compared their fruiting and branch architecture features to determine the possibility to use ‘Calatina’ olive trees for intensive plantings, as a local alternative to the international reference ‘Arbequina’. The study indicated that ‘Calatina’ is more efficient in terms of yield and harvesting than ‘Arbequina’. This qualifies ‘Calatina’ as a superior, yield-efficient olive cultivar suitable for intensive hedgerow plantings to be harvested with straddle or side-by-side trunk shaker machines. Sirgedaitė-Šėžienė et al. [6] used ‘Rubin’ apple trees grafted on dwarfing P60 rootstocks to determine the impact of canopy training treatments as a stress factor on metabolic response to obtain key information on how to improve physiological behaviour and the management of growth and development of apple trees. The results indicated that all applied canopy training treatments significantly increased the total phenol and total starch contents in apple tree leaves. Scalisi et al. [7] in their work aimed to derive a new fruit skin colour attribute—namely, a Colour Development Index (CDI), ranging from 0 to 1, that intuitively increases as fruit becomes redder—to assess colour development in peach and nectarine fruit skin. The study found that the CDI can serve as a standardised and objective skin colour index for peaches and nectarines. Čirjak et al. [8] summarize the automatic methods (image analysis systems, smart traps, sensors, decision support systems, etc.) used to monitor the major pest in apple production (Cydia pomonella L.) and other important apple pests and fruit flies to improve sustainable pest management under frequently changing climatic conditions. Pisciotta et al. [9] released a review underlying the opportunities offered by the recently developed table-grape soil-less cultivation systems; this is an up-to-date examination of the latest experimental and applied findings of the sector’s research activities. A special emphasis is given to the evolution of the applied technical solutions, varietal choice, and environmental conditions for the aims of table-grape soil-less cultivation. Borgogno-Mondino et al. [10] evaluated a promising alternative offered by Copernicus Sentinel 2 data (S2) to midday stem water potential for monitoring the water status of pomegranate plants and for addressing irrigation management. Despite limited ground observations, the results showed the promising capability of spectral indices (NDVI, NDRE, and NDWI) and S2 bands in estimating Ψstem readings. Boini et al. [11], in their study, used shading nets to lower irrigation requirements and make apple growing more sustainable. The encouraging results showed a comparable yield and fruit quality saving 50% of irrigation water under a classic anti-hail system compared to the control treatment

Testing the Short-Term Efficacy of a Lipid-Lowering Nutraceutical in the Setting of Clinical Practice: A Multicenter Study

The main guidelines for cardiovascular disease prevention suggest that nutraceuticals could be an efficacious tool to improve lipid pattern. Our aim was to carry out a clinical trial comparing the metabolic effects of a combined nutraceutical containing both red yeast rice and polyunsaturated fatty acids (PUFAs) and a phytosterol-based approach in a setting of clinical practice. This was a multicenter open study with parallel control. We consecutively enrolled 107 pharmacologically untreated subjects affected by primary polygenic hypercholesterolemia and metabolic syndrome, assigned to 8-week treatment with a combined treatment with red yeast rice (Dif1Stat\uc2\uae, including 5mg monacolin K) and 610mg PUFAs. A parallel group of 30 subjects with similar characteristics was treated with phytosterols 1600mg/die. In the combined nutraceutical group, compared with the baseline level, we observed a significant decrease in total cholesterol (TC; -42.50\uc2\ub118.1mg/dL), low-density lipoprotein cholesterol (LDL-C; -37.6\uc2\ub113.6mg/dL), triglycerides (TG; -19.8\uc2\ub125.1mg/dL), and non-HDL-C (-43.1\uc2\ub117.7mg/dL) (all P<.001). In the phytosterol-treated group, compared to the baseline level, we observed a significant decrease in TC (-13.7\uc2\ub14.3mg/dL), LDL-C (-17.6\uc2\ub18.5mg/dL), and non-HDL-C (-14.1\uc2\ub15.6mg/dL) (all P<.001). When comparing the combined nutraceutical effect with that of phytosterols, we observed that the combined nutraceutical intake was associated with a significantly higher decrease in TC, LDL-C, TG, and non-HDL-C (all P<.001). In the short term, a combined nutraceutical containing red yeast rice and PUFAs is well tolerated and efficacious in reducing plasma lipid levels in subjects affected by primary polygenic hypercholesterolemia and metabolic syndrome

Evaluation of graft success of grapevine after incubation room by means of thermographic, electrical and mechanical techniques

Grafting is an important technique for getting good yields in plant multiplication. Understanding the success of the graft after the incubation stage is important to the evaluation of the suitable grafting for the open field (nursery). Successful grafting in vines requires the development of a functional vascular system between the scion and the rootstock. The graft compatibility and its augmentation depend upon various natural factors like environment, soil conditions and protective measures. The present study examines the capability of thermographic, mechanical and electrical techniques to assess the graft quality and success after the incubation stage. The trial was carried out at Vivai Mannone, (Petrosino, Western Sicily, 37\uc2\ub042'26.28''N - 12\uc2\ub029'09.57''E). After the different tests, various grafting combinations were planted in the nursery and followed for the vegetative season. Before the evaluation procedure was performed graft unions have been subjected to a moderate heating from ambient temperature, then the thermal transient toward ambient temperature was monitored by means of a thermal imaging camera. As far as the electrical testing procedure was concerned, a sinusoidal voltage was applied through the grafts-cuttings, and the voltage attenuation at different points at increasing distances from the source was measured by an oscilloscope. The mechanical strength of the graft undergoing a controlled rate flexural loading was monitored by a PC remote controlled digital dynamometer. Experimental results show that we were able to distinguish the successful grafting only with the thermographic test. Moreover, this technique was the only non-destructive test from which it was possible to derive quantitative parameters, useful to provide successful nursery forecast. Engraftment results detected at the nursery showed a 15% error in forecast based on the proposed thermal image method, which is a satisfactory value for a feasibility study

The combination of DInSAR and facility damage data for the updating of slow-moving landslide inventory maps at medium scale

Abstract. Testing innovative procedures and techniques to update landslide inventory maps is a timely topic widely discussed in the scientific literature. In this regard remote sensing techniques – such as the Synthetic Aperture Radar Differential Interferometry (DInSAR) – can provide a valuable contribution to studies concerning slow-moving landslides in different geological contexts all over the world. In this paper, DInSAR data are firstly analysed via an innovative approach aimed at enhancing both the exploitation and the interpretation of remote sensing information; then, they are complemented with the results of an accurate analysis of survey-recorded damage to facilities due to slow-moving landslides. In particular, after being separately analysed to provide independent landslide movement indicators, the two datasets are combined in a DInSAR-Damage matrix which can be used to update the state of activity of slow-moving landslides. Moreover, together with the information provided by geomorphological maps, the two datasets are proven to be useful in detecting unmapped phenomena. The potentialities of the adopted procedure are tested in an area of southern Italy where slow-moving landslides are widespread and accurately mapped by using geomorphological criteria

Application of SINTACS method to the aquifers of Piana di Palermo, Siciliy,Italy

Piana di Palermo is characterized by the presence of two different aquifers: one shallow, constituted by calcarenites and the other deep in fractured carbonates. The calcarenitic aquifer presents a low potential compared to the carbonatic aquifer. The chemistry of the water in both aquifers reflects water-rock interaction, seawater intrusion, ionic exchange etc. The application of SINTACS method showed that about 80% of the study area can be classified as of high to very high vulnerability.Piana di Palermo es caracterizada por la presencia de dos acuíferos diferentes: uno somero constituido por calcarenitas y otro profundo en rocas carbonatadas fracturadas. El acuífero calcarenítico presenta un bajo potencial hídrico comparado con el acuífero carbonático. El comportamiento químico del agua en ambos acuíferos refleja severos procesos como interacción agua-roca, intrusión marina, cambio iónico, etc. La aplicación del método SINTACS mostró que cerca del 80 % del área en estudio puede ser clasificada como de alta a muy alta vulnerabilidad

Use of confocal microscopy imaging for in vitro assessment of adipose-derived mesenchymal stromal cells seeding on acellular dermal matrices: 3D reconstruction based on collagen autofluorescence

Background: Both mesenchymal stromal cells (MSCs) and acellular dermal matrices (ADMs) represent fascinating therapeutic tools in the wound healing scenario. Strategies aimed at combining these two treatment modalities are currently under investigation. Moreover, scarcity of quantitative, nondestructive techniques for quality assessment of engineered tissues poses great limitations in regenerative medicine and collagen autofluorescence-based imaging techniques are acquiring great importance in this setting. Objective: Our goals were to assess the in vitro interactions between ADSCs and ADMs and to analyze extracellular-matrix production. Methods: Adipose-derived MSCs (ADSC) were plated on 8-mm punch biopsies of a commercially available ADM (Integra\uae). Conventional histology with hematoxylin-eosin staining, environmental scanning electron microscopy, and confocal-laser scanning microscopy were used to obtain imaging of ADSC-seeded ADMs. Collagen production by ADSCs was quantified by mean fluorescence intensity (MFI), expressed in terms of positive pixels/field, obtained through ImageJ software processing of three-dimensional projections from confocal scanning images. Control conditions included: fibroblast-seeded ADM, ADSC- and fibroblast-induced scaffolds, and Integra\uae alone. Results: ADSCs were efficiently seeded on Integra\uae and were perfectly incorporated in the pores of the scaffold. Collagen production was revealed to be significantly higher when ADSCs were seeded on ADM rather than in all other control conditions. Collagen autofluorescence was efficiently used as a surrogate marker of ECM production. Conclusions: Combined therapies based on MSCs and collagenic ADMs are promising therapeutic options for chronic wounds. Not only ADSCs can be efficiently seeded on ADMs, but ADMs also seem to potentiate their regenerative properties, as highlightable from fluorescence confocal imaging

Poorly differentiated clusters (PDC) in colorectal cancer: Does their localization in tumor matter?

Poorly differentiated clusters (PDC) are aggregates of at least five neoplastic cells lacking evidence of glandular differentiation. By definition, they can be present at the invasive front (peripheral PDC or pPDC) and within the tumor stroma (central PDC or cPDC). In colorectal cancer (CRC), PDC are considered adverse prognosticators and seem to reflect epithelial mesenchymal transition (EMT). In this study, we have investigated the immuno-expression of two EMT-related proteins, E-cadherin and β-catenin, in PDC of primary CRCs and matched liver metastases. pPDC always showed nuclear β-catenin staining and diffusely reduced/absence of E-cadherin expression as opposed cPDC which showed nuclear β-catenin immunoreactivity and E-cadherin expression in about 50% of cases. In addition, the pattern of β-catenin and E-cadherin expression differed between PDC and the main tumor, and between primary CRC and liver metastasis (LM), in a percentage of cases. A discordant pattern of β-catenin and E-cadherin expression between pPDC and cPDC, between main tumor and cPDC, and between primary CRC and LM, confirms that EMT is a dynamic and reversible process in CRC. On the overall, this suggests that pPDC and cPDC are biologically different. We may advocate that PDC develop at the tumor center (cPDC) and then some of them migrate towards the tumor periphery while progressively completing EMT process (pPDC). Based on these results, PDC presence and counting may have different prognostic relevance if the assessment is done at the invasive front of the tumor or in the intratumor stroma

Titanium Surface Properties Influence the Biological Activity and FasL Expression of Craniofacial Stromal Cells.

Mesenchymal stromal cells (MSCs) can be easily isolated form craniofacial bones during routine dentistry procedures. Due to their embryological origin from neural crest, they represent a suitable cell population to study cell-biomaterial interaction in the craniofacial field, including osteoinductive/osteointegrative processes. The biological and immunomodulatory properties of MSCs may be influenced by chemistry and topography of implant surfaces. We investigated if and how three different titanium surfaces, machined (MCH), sandblasted with resorbable blasting medium (RBM), and Ca++-nanostructured (NCA), may affect biological activity, osseointegration, and immunomodulatory properties of craniofacial MSCs. Cell proliferation, morphology, osteogenic markers, and FasL were evaluated on MSCs isolated from the mandibular bone after seeding on these three different surfaces. No statistically significant differences in cell proliferation were observed whereas different morphologies and growth patterns were detected for each type of surface. No difference in the expression of osteogenic markers was revealed. Interestingly, FasL expression, involved in the immunomodulatory activity of stem cells, was influenced by surface properties. Particularly, immunofluorescence analysis indicated that FasL expression increased on MCH surface compared to the others confirming the suggested role of FasL in promoting osteogenic differentiation. Titanium surface treatments and topography might reflect different biological behaviours of craniofacial MSCs and influence their osseointegration/immunomodulation properties